10.17863/CAM.10697
Stanley, Elizabeth G
Jenkins, Benjamin J
Walker, Celia G
Koulman, Albert
0000-0001-9998-051X
Browning, Lucy
West, Annette L
Calder, Philip C
0000-0002-6038-710X
Jebb, Susan A
Griffin, Julian
0000-0003-1336-7744
Lipidomics Profiling of Human Adipose Tissue Identifies a Pattern of Lipids Associated with Fish Oil Supplementation.
Apollo - University of Cambridge Repository (staging)
2017
Adipose Tissue
Animals
Humans
Body Weight
Docosahexaenoic Acids
Eicosapentaenoic Acid
Fatty Acids, Nonesterified
Fatty Acids, Omega-6
Triglycerides
Phospholipids
Fish Oils
Case-Control Studies
Principal Component Analysis
Dietary Supplements
Adult
Middle Aged
Female
Male
Lipid Metabolism
Apollo - University of Cambridge Repository (staging)
Apollo - University of Cambridge Repository (staging)
2018-06-21
2018-06-21
2017-09
Article
1535-3893
https://www.repository.cam.ac.uk/handle/1810/277303
1535-3907
To understand the interaction between diet and health, biomarkers that accurately reflect consumption of foods of perceived health relevance are needed. The aim of this investigation was to use direct infusion-mass spectrometry (DI-MS) lipidomics to determine the effects of fish oil supplementation on lipid profiles of human adipose tissue. Adipose tissue samples from an n-3 polyunsaturated fatty acid (PUFA) supplementation study (n = 66) were analysed to compare the pattern following supplementation equivalent to 0 or 4 portions of oily fish per week. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were incorporated into highly unsaturated (≥5 double bonds) triglycerides (TGs), phosphocholines and phosphoethanolamines, as well as being detected directly as the non-esterified fatty acid forms. Multivariate statistics demonstrated that phospholipids were the most accurate and sensitive lipids for the assessing EPA and DHA incorporation into adipose tissue. Potential confounding factors (adiposity, age and sex of the subject) were also considered in the analysis, and adiposity was also associated with an increase in highly unsaturated TGs as a result of incorporation of the n-6 PUFA arachidonic acid. DI-MS provides a high throughput analysis of fatty acid status that can monitor oily fish consumption, suitable for use in cohort studies.
Wellcome Trust
093148/Z/10/Z
Medical Research Council
MR/P01836X/1
Biotechnology and Biological Sciences Research Council
BB/H013539/2
Biotechnology and Biological Sciences Research Council
BB/H013539/1
Biotechnology and Biological Sciences Research Council
BB/P028195/1
Medical Research Council
MR/P011705/1
Biotechnology and Biological Sciences Research Council
BB/M027252/2