10.17863/CAM.6634
Bongiovanni, Marie
Godet, J
Horrocks, Mathew
Tosatto, Laura
Carr, Alexander
Wirthensohn, David
0000-0003-4403-9436
Ranasinghe, Rohan
0000-0001-9227-4110
Fritz, JV
Dobson, Christopher
Klenerman, David
0000-0001-7116-6954
Lee, Steven
0000-0003-4492-5139
Multi-dimensional super-resolution imaging enables surface hydrophobicity mapping
DSpace at Cambridge (dev)
2016
DSpace at Cambridge (dev)
DSpace at Cambridge (dev)
2016-12-06
2016-12-06
en
Article
2041-1723
https://www.repository.cam.ac.uk/handle/1810/261446
2041-1723
10.1038/ncomms13544
10.17863/CAM.6463
Attribution 4.0 International
We have developed a multi-dimensional super-resolution (md-SR) imaging technique to determine both the localization and the environmental properties of single-molecule fluorescent emitters. The method, termed sPAINT, exploits the solvatochromic and fluorogenic properties of nile red to extract both the emission spectrum and the position of each dye molecule to enable the mapping of hydrophobicity of biological structures. We first validated the sPAINT method by studying synthetic lipid vesicles of known composition, then applied it to measure the hydrophobicity of amyloid fibrils and oligomers implicated in neurodegenerative diseases, and of the plasma membrane of mammalian cells. sPAINT is easily implemented by inserting a transmission diffraction grating into the optical path of a localization-based super-resolution microscope, which enables all the necessary information to be extracted simultaneously from a single image plane. sPAINT enables the hydrophobicity of surfaces to be mapped at the nanoscale in a dynamic fashion.
Medical Research Council (Grant ID: MR/K015850/1), Engineering and Physical Sciences Research Council, Royal Society (University Research Fellowship, Grant ID: UF120277), Augustus Newman Foundation, Cambridge Advanced Imaging Centre, Christ’s College
Royal Society
uf120277
MRC
MR/K015850/1