10.25358/DSPACE-DEV-86492
Neufert, Clemens
Becker, Christoph
Neurath, Markus F.
An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression
Johannes Gutenberg-Universität Mainz
2007
610 Medizin
610 Medical sciences Medicine
Johannes Gutenberg-Universität Mainz
Johannes Gutenberg-Universität Mainz
2020-03-12
2020-03-12
2007
Nature protocols. Bd. 2. H. 8. Basingstoke : Nature Publishing Group. S. 1998 - 2004
https://dspace-dev.ub.uni-mainz.de/handle/20.500.12030/139543
Colorectal cancer is a life-threatening disease that can develop spontaneously or as a complication of inflammatory bowel diseases. Mouse models are essential tools for the preclinical testing of novel therapeutic options in vivo. Here, we provide a highly reliable protocol for an experimental mouse model to study the development of colon cancers. It is based on the mutagenic agent azoxymethane (AOM), which exerts colonotropic carcinogenicity. Repeated intraperitoneal administration of AOM results in the development of spontaneous tumors within 30 weeks. As an alternative option, inflammation-dependent tumor growth can be investigated by combining the administration of AOM with the inflammatory agent dextran sodium sulfate in drinking water, which causes rapid growth of multiple colon tumors per mouse within 10 weeks. Different scoring systems including number of tumors and tumor size identify factors promoting or inhibiting tumor initiation and/or tumor progression, respectively.