10.7272/FXCF-BP60
Greninger, Alexander
DeRisi, Joseph
Pooled metagenomic sequence from human stool
University of California, San Francisco
2014
Metagenomics
Viral sequence
Klassevirus
UCSF
2014-06-12T21:39:53+00:00
Dataset
UCSF Datashare Data Use Agreement
This dataset corresponds to the data presented in "The complete
genome of klassevirus – a novel picornavirus in pediatric stool" by
Greninger et al (http://www.ncbi.nlm.nih.gov/pubmed/19538752).
141 diarrhea samples were prepared, pooled, and pyrosequenced on the Roche
Genome Sequence FLX as described in "The complete genome of
klassevirus – a novel picornavirus in pediatric stool" by Greninger
et al (http://www.ncbi.nlm.nih.gov/pubmed/19538752). A novel member of the
Picornaviridae viral family, Human klassevirus 1, was discovered in the
540,412 unique reads with an average length of 240 nt. The reads were
stripped of sequences mapping with 90% or greater identity to known
diarrhea-causing viruses: 6,959 reads mapped to the Adenoviridae and
Caliciviridae viral families by translated BLAST. Of the remaining 533,453
unique reads, 483 derived from Human klassevirus 1. The data are provided
in FASTA format.
The complete genome of klassevirus a novel picornavirus in pediatric
stool. Greninger AL, Runckel C, Chiu CY, Haggerty T, Parsonnet J, Ganem D,
DeRisi JL. Virol J. 2009 Jun 18;6:82. doi: 10.1186/1743-422X-6-82. PMID:
19538752