10.80001/1-4TKC
Paul Thomas
Paul
Thomas
Pedro Sanchez
Pedro
Sanchez
Yang Chai
Yang
Chai
Whole-genome transcriptome profiling of E12.5 Wnt1-Cre;Tgfbr2flfl by microarray of maxilla and mandible
We performed the global gene expression profiling analysis of the proximal (P ) and distal (D) regions of the mandible and proximal (P ) and distal (D) regions of the maxilla using microarray. Total RNA samples (1 ug per sample) were converted into biotin-labeled cRNA using the GeneChip® IVT Labeling Kit and standard protocols recommended by Affymetrix (Santa Clara, CA). Fragmented cRNA was applied to GeneChip, Mouse Genome 430 2.0 Arrays (Affymetrix) that contain probe sets designed to detect over 39,000 transcripts. Microarrays were hybridized, processed and scanned, as previously described using manufacturers recommended conditions.
FaceBase (www.facebase.org)
2020
transcription profiling by array assay
Dataset
Craniofacial development
Craniosafial malformation
en
Dataset
1
National Institute of Health (NIH)
5U01DE024449
FaceBase Coordinating Center