10.80001/VHR
Pedro A. Sanchez
Pedro
Sanchez
Richard C. Pelikan
Richard
Pelikan
Yang Chai
Yang
Chai
Gene expression profiling of palatal and mandibular tissue in Msx1-/- mice
We performed global gene expression analyses of the palatal and palatal tissue of Msx1-/- and control mice at embryonic day E14.5 Total RNA samples (1 ug per sample) were converted into biotin-labeled cDNA using the Enzo BioArray Terminal Labeling Kit with Biotin-ddUTP and standard protocols recommended by Affymetrix (Santa Clara, CA). Fragmented cDNA was applied to GeneChip, Mouse Genome 430 2.0 Arrays (Affymetrix) that contain probe sets designed to detect over 39,000 transcripts. Microarrays were hybridized, processed and scanned, as previously described using manufacturers recommended conditions.
FaceBase (www.facebase.org)
2020
transcription profiling by array assay
Dataset
Craniofacial development
Craniosafial malformation
en
Dataset
1
National Institute of Health (NIH)
5U01DE024449
FaceBase Coordinating Center